E5144
 |  |  |
 | The specific recognition sequence for enterokinase |
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E5144
Sigma
Enterokinase from bovine intestine
powder
| Synonym: | Enteropeptidase |
| CAS Number: | 9014-74-8 |
| Enzyme Commission (EC) Number: | 3.4.21.9 ( BRENDA | IUBMB ) |
| EC Number: | 232-761-1 |
| MDL number: | MFCD00131020 |
Description
| Application | Removes FLAG peptide from N-terminal and Met-N-terminal fusion proteins. |
| Enterokinase is a member of the S1 peptidase family. In vivo, it is responsble for the proteolytic activation of trypsin from trypsinogen. Enterokinase is used for site specific cleavage of recombinant fusion proteins containing an accessible enterokinase recognition site for removal of affinity tags. | |
| Packaging | Supplied with optimized enterokinase buffer |
Properties
| form | powder |
| color | white |
| shipped in | dry ice |
| storage temp. | −20°C |
Safety
| Personal Protective Equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
| WGK Germany | 3 |
Related Products
| Substrate | G5261, Gly-Asp-Asp-Asp-Asp-Lys-β-naphthylamide |
